Pranav took overstretch data sets using 0.5x popping buffer with regular H2O and also making 0.5x popping buffer by adding D2O to 1x popping buffer. The data sets are in * H2OThu, July 14, 2011 01:37:10 PM Data File Path \\Controller\users\rathi.pranav\Desktop\110714\0478.dat Header File Path\\Controller\users\rathi.pranav\Desktop\110714\0478.ini OVERSTRETCHING SAMPLE H2O;.002 * D2OThu, July 14, 2011 02:12:25 PM Data File Path \\Controller\users\rathi.pranav\Desktop\110714\0479.dat Header File Path\\Controller\users\rathi.pranav\Desktop\110714\0479.ini OVERSTRETCHING SAMPLE D2O;.002 Images of converted (preliminary calibration, no offset, etc.) can be found here:http://kochlab.org/files... Initially I went through and picked these data sets by eye, not paying attention to the overall force difference between overstretch, but just looking for things without sticking, breaking, and reasonably "flat"... - Steve Koch